diff --git a/src/lfx/pyproject.toml b/src/lfx/pyproject.toml
index e705c6339c3a..a2b7b26bfb50 100644
--- a/src/lfx/pyproject.toml
+++ b/src/lfx/pyproject.toml
@@ -19,7 +19,7 @@ dependencies = [
     "pillow>=12.3.0,<13.0.0",
     "fastapi>=0.135.0,<1.0.0",
     # [standard] adds uvloop + httptools (fast loop + HTTP parser) for the `lfx serve` worker path.
-    "uvicorn[standard]>=0.34.3,<1.0.0",
+    "uvicorn[standard]>=0.45.0,<1.0.0",
     # gunicorn powers `lfx serve --workers N` on Unix; Windows falls back to uvicorn and rejects gunicorn-only flags.
     "gunicorn>=22.0; sys_platform != 'win32'",
     # a2wsgi bridges the ASGI app onto gunicorn's sync worker for `lfx serve --use-sync-workers` (Unix).
diff --git a/src/lfx/src/lfx/cli/commands.py b/src/lfx/src/lfx/cli/commands.py
index 9a917ba779a7..d6dbe6beeb6a 100644
--- a/src/lfx/src/lfx/cli/commands.py
+++ b/src/lfx/src/lfx/cli/commands.py
@@ -561,7 +561,18 @@ def serve_command(
                 os.environ[_SERVE_RESET_ENVIRON_ENV] = "1" if reset_environ else "0"
                 try:
                     serve_app = create_multi_serve_app(registry=registry, identity_config=identity_config)
-                    uvicorn.run(serve_app, host=host, port=port, workers=1, log_level=log_level)
+                    uvicorn.run(
+                        serve_app,
+                        host=host,
+                        port=port,
+                        workers=1,
+                        log_level=log_level,
+                        # Start every request task from a clean context, or a pipelined request
+                        # inherits the previous request's ended server span and its own span is
+                        # emitted as an INTERNAL child of an unrelated request. See
+                        # ``LFXUvicornWorker.CONFIG_KWARGS`` for the mechanism.
+                        reset_contextvars=True,
+                    )
                 finally:
                     # Symmetry with _launch_workers: don't leave our key in the parent env.
                     os.environ.pop(_SERVE_RESET_ENVIRON_ENV, None)
@@ -717,6 +728,8 @@ def _launch_workers(
                 workers=workers,
                 log_level=log_level,
                 factory=True,
+                # Same reason as the single-worker path above.
+                reset_contextvars=True,
             )
         else:
             # gunicorn ships with lfx on Linux/macOS; a missing import means a trimmed/broken
diff --git a/src/lfx/src/lfx/cli/serve_gunicorn.py b/src/lfx/src/lfx/cli/serve_gunicorn.py
index 3de47c157b47..1ae2f5f28dd6 100644
--- a/src/lfx/src/lfx/cli/serve_gunicorn.py
+++ b/src/lfx/src/lfx/cli/serve_gunicorn.py
@@ -26,6 +26,22 @@ class LFXUvicornWorker(UvicornWorker):
     here and can host per-worker customization if needed later.
     """
 
+    # ``reset_contextvars``: start every request task from a clean context. A request that
+    # arrives while another is still in flight on the same connection is queued as a pipelined
+    # request, and uvicorn then starts it from inside the finishing request's task
+    # (httptools_impl ``on_response_complete`` -> ``_start_asgi_task``). ``create_task`` copies
+    # the context, so the new request begins with the previous request's already-ended server
+    # span still current. OpenTelemetry's ASGI middleware reads that as nesting and emits the
+    # request as an INTERNAL child of an unrelated, finished request instead of as a SERVER
+    # root, which merges unrelated traces and hides most HTTP traffic from RED metrics and
+    # service maps. A browser page load triggers it; sequential curl never does. See CPython
+    # #140947.
+    #
+    # Spread over the base class's kwargs rather than replacing them, so the inherited loop and
+    # http choices still apply. Kept in step with the two ``uvicorn.run`` calls in
+    # ``lfx.cli.commands``, and with ``langflow.server.LangflowUvicornWorker``.
+    CONFIG_KWARGS = {**UvicornWorker.CONFIG_KWARGS, "reset_contextvars": True}
+
 
 class LFXGunicornApp(BaseApplication):
     def __init__(self, app_import_string: str, options: dict) -> None:
diff --git a/src/lfx/tests/unit/cli/test_serve_request_context_isolation.py b/src/lfx/tests/unit/cli/test_serve_request_context_isolation.py
new file mode 100644
index 000000000000..086e2f5676b9
--- /dev/null
+++ b/src/lfx/tests/unit/cli/test_serve_request_context_isolation.py
@@ -0,0 +1,73 @@
+"""Every ``lfx serve`` launch path must start each request task from a clean context.
+
+A request that arrives while another is still in flight on the same connection is queued as a
+pipelined request, and uvicorn starts it from inside the finishing request's task
+(``httptools_impl.on_response_complete`` -> ``_start_asgi_task``). ``create_task`` copies the
+context, so without ``reset_contextvars`` the new request begins with the previous request's
+already-ended server span still current. OpenTelemetry's ASGI middleware reads that as nesting
+and emits the request as an INTERNAL child of an unrelated finished request rather than as a
+SERVER root, merging unrelated traces and hiding most HTTP traffic from RED metrics.
+
+langflow set the flag on both of its launch paths; lfx serve has three and had none of them,
+so the same defect stayed live on the runtime that actually serves traffic. These tests cover
+all three, and the source-level one covers a fourth if anyone adds it.
+"""
+
+from __future__ import annotations
+
+import ast
+from pathlib import Path
+
+import lfx.cli.commands
+import pytest
+import uvicorn
+
+
+def test_the_gunicorn_worker_resets_the_context_and_keeps_its_inherited_options():
+    """Asserted through a real ``uvicorn.Config``, so a renamed or rejected option fails here.
+
+    The inherited keys matter too: ``CONFIG_KWARGS`` is spread over the base class's, and
+    replacing it outright would silently drop uvicorn's loop and http selection.
+    """
+    pytest.importorskip("gunicorn", reason="the gunicorn worker path is Unix-only")
+
+    from lfx.cli.serve_gunicorn import LFXUvicornWorker
+    from uvicorn.workers import UvicornWorker
+
+    config = uvicorn.Config("lfx.cli.serve_app:create_serve_app", **LFXUvicornWorker.CONFIG_KWARGS)
+
+    assert config.reset_contextvars is True
+    for key, value in UvicornWorker.CONFIG_KWARGS.items():
+        assert LFXUvicornWorker.CONFIG_KWARGS[key] == value, f"dropped inherited {key}"
+
+
+def _uvicorn_run_calls() -> list[ast.Call]:
+    source = Path(lfx.cli.commands.__file__).read_text(encoding="utf-8")
+    return [
+        node
+        for node in ast.walk(ast.parse(source))
+        if isinstance(node, ast.Call)
+        and isinstance(node.func, ast.Attribute)
+        and node.func.attr == "run"
+        and isinstance(node.func.value, ast.Name)
+        and node.func.value.id == "uvicorn"
+    ]
+
+
+def test_every_uvicorn_launch_in_the_serve_command_resets_the_context():
+    """Read from the source rather than by starting a server or patching ``uvicorn.run``.
+
+    This is the guard that would have caught the original gap. Both call sites are literal
+    kwargs, and a new launch path added later fails here until it opts in too.
+    """
+    calls = _uvicorn_run_calls()
+    assert len(calls) >= 2, "expected the single-worker and Windows multi-worker launches"
+
+    for call in calls:
+        passed = {kw.arg: kw.value for kw in call.keywords}
+        assert "reset_contextvars" in passed, f"uvicorn.run at line {call.lineno} does not reset the context"
+        value = passed["reset_contextvars"]
+        assert isinstance(value, ast.Constant), (
+            f"uvicorn.run at line {call.lineno} passes a non-literal reset_contextvars={ast.unparse(value)}"
+        )
+        assert value.value is True, f"uvicorn.run at line {call.lineno} passes reset_contextvars={value.value!r}"
diff --git a/uv.lock b/uv.lock
index 7e8cf8dc3cfa..883cfaccea36 100644
--- a/uv.lock
+++ b/uv.lock
@@ -9297,7 +9297,7 @@ requires-dist = [
     { name = "tomli", specifier = ">=2.2.1,<3.0.0" },
     { name = "typer", specifier = ">=0.16.0,<1.0.0" },
     { name = "typing-extensions", specifier = ">=4.14.0,<5.0.0" },
-    { name = "uvicorn", extras = ["standard"], specifier = ">=0.34.3,<1.0.0" },
+    { name = "uvicorn", extras = ["standard"], specifier = ">=0.45.0,<1.0.0" },
     { name = "validators", specifier = ">=0.34.0,<1.0.0" },
     { name = "wheel", specifier = ">=0.46.2,<1.0.0" },
 ]