diff --git a/CHANGELOG.md b/CHANGELOG.md
index 0963f2e09..0a2c0010f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -55,6 +55,7 @@ Special thanks to the following for their contributions to the release:
 - [PR #1725](https://github.com/nf-core/rnaseq/pull/1725) - Refine .nftignore patterns to reinstate tx2gene MD5 checking, remove redundant UNTAR version collection
 - [PR #1728](https://github.com/nf-core/rnaseq/pull/1728) - Re-render metro map with nf-metro v0.5.4: bolder section labels and number badges for improved visual hierarchy, increased vertical spacing between stacked sections, synchronized animation timing
 - [PR #1730](https://github.com/nf-core/rnaseq/pull/1730) - Bump Nextflow from 25.04.0 to 25.04.3 in CI to fix `conda create --mkdir` failure ([nextflow-io/nextflow#5947](https://github.com/nextflow-io/nextflow/issues/5947))
+- [PR #1734](https://github.com/nf-core/rnaseq/pull/1734) - Compose `test_prokaryotic` profile on `prokaryotic` to remove duplicated settings
 - [PR #1735](https://github.com/nf-core/rnaseq/pull/1735) - Code quality improvements: replace opaque tuple indexing with named destructuring, rename `ch_dummy_file`, move metro map docs, align GPU CI workflow with other workflows
 
 ### Parameters
diff --git a/conf/test_prokaryotic.config b/conf/test_prokaryotic.config
index e2db29fa8..549b73c1b 100644
--- a/conf/test_prokaryotic.config
+++ b/conf/test_prokaryotic.config
@@ -8,6 +8,9 @@
     Use as follows:
         nextflow run nf-core/rnaseq -profile test_prokaryotic,<docker/singularity> --outdir <OUTDIR>
 
+    Note: test_prokaryotic composes on the prokaryotic profile (via nextflow.config),
+    so core prokaryotic settings are inherited automatically.
+
 ----------------------------------------------------------------------------------------
 */
 
@@ -33,22 +36,6 @@ params {
     transcript_fasta = null
     additional_fasta = null
 
-    // Prokaryotic-specific settings
-    prokaryotic              = true
-    gffread_transcript_fasta = true
-    featurecounts_feature_type = 'CDS'
-
-    // Default aligner for prokaryotes (can be overridden with --aligner star_salmon)
-    aligner = 'bowtie2_salmon'
-
-    // Skip bigwig for bowtie2_salmon (aligns to transcriptome, not genome)
-    skip_bigwig = true
-
-    // Skip QC tools that assume eukaryotic features
-    skip_rseqc    = true
-    skip_dupradar = true
-    skip_qualimap = true
-
     // Skip steps not needed for minimal testing
     skip_bbsplit       = true
     skip_preseq        = true
diff --git a/nextflow.config b/nextflow.config
index 9e77da5c5..f60fdf0df 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -288,7 +288,10 @@ profiles {
         }
     }
     test_full        { includeConfig 'conf/test_full.config'        }
-    test_prokaryotic { includeConfig 'conf/test_prokaryotic.config' }
+    test_prokaryotic {
+        includeConfig 'conf/prokaryotic.config'
+        includeConfig 'conf/test_prokaryotic.config'
+    }
     prokaryotic      { includeConfig 'conf/prokaryotic.config'      }
     test_full_aws {
         includeConfig 'conf/test_full.config'