diff --git a/tower-http/src/services/fs/serve_dir/future.rs b/tower-http/src/services/fs/serve_dir/future.rs
index 7df32b0d..3712c055 100644
--- a/tower-http/src/services/fs/serve_dir/future.rs
+++ b/tower-http/src/services/fs/serve_dir/future.rs
@@ -13,6 +13,7 @@ use http::{
     HeaderValue, Request, Response, StatusCode,
 };
 use http_body_util::{BodyExt, Empty, Full};
+use http_range_header::RangeUnsatisfiableError;
 use pin_project_lite::pin_project;
 use std::{
     convert::Infallible,
@@ -297,6 +298,14 @@ fn build_response(output: FileOpened) -> Response<ResponseBody> {
             }
         }
 
+        Some(Err(RangeUnsatisfiableError::OverlappingRanges)) => builder
+            .header(header::CONTENT_RANGE, format!("bytes */{}", size))
+            .status(StatusCode::RANGE_NOT_SATISFIABLE)
+            .body(body_from_bytes(Bytes::from(
+                "Cannot serve multipart range requests",
+            )))
+            .unwrap(),
+
         Some(Err(_)) => builder
             .header(header::CONTENT_RANGE, format!("bytes */{}", size))
             .status(StatusCode::RANGE_NOT_SATISFIABLE)
diff --git a/tower-http/src/services/fs/serve_dir/open_file.rs b/tower-http/src/services/fs/serve_dir/open_file.rs
index 9dd1e012..fbe67265 100644
--- a/tower-http/src/services/fs/serve_dir/open_file.rs
+++ b/tower-http/src/services/fs/serve_dir/open_file.rs
@@ -309,8 +309,15 @@ fn try_parse_range(
     file_size: u64,
 ) -> Option<Result<Vec<RangeInclusive<u64>>, RangeUnsatisfiableError>> {
     maybe_range_ref.map(|header_value| {
-        http_range_header::parse_range_header(header_value)
-            .and_then(|first_pass| first_pass.validate(file_size))
+        let parsed = http_range_header::parse_range_header(header_value)?;
+
+        if parsed.ranges.len() > 1 {
+            // ServeDir/ServeFile do not support multipart responses, so reject
+            // multi-range requests before validate() runs overlap checks.
+            return Err(RangeUnsatisfiableError::OverlappingRanges);
+        }
+
+        parsed.validate(file_size)
     })
 }
 
diff --git a/tower-http/src/services/fs/serve_dir/tests.rs b/tower-http/src/services/fs/serve_dir/tests.rs
index e220197a..1896ea67 100644
--- a/tower-http/src/services/fs/serve_dir/tests.rs
+++ b/tower-http/src/services/fs/serve_dir/tests.rs
@@ -627,6 +627,27 @@ async fn read_partial_errs_on_bad_range() {
     )
 }
 
+#[tokio::test]
+async fn multipart_range_valid_returns_multipart_error_body() {
+    let svc = ServeDir::new("..");
+    let req = Request::builder()
+        .uri("/README.md")
+        .header("Range", "bytes=0-0,2-2")
+        .body(Body::empty())
+        .unwrap();
+    let res = svc.oneshot(req).await.unwrap();
+
+    assert_eq!(res.status(), StatusCode::RANGE_NOT_SATISFIABLE);
+    let file_contents = std::fs::read("../README.md").unwrap();
+    assert_eq!(
+        res.headers()["content-range"],
+        &format!("bytes */{}", file_contents.len())
+    );
+
+    let body = body_into_text(res.into_body()).await;
+    assert_eq!(body, "Cannot serve multipart range requests");
+}
+
 #[tokio::test]
 async fn accept_encoding_identity() {
     let svc = ServeDir::new("..");