If the population size is overridden using -n, the 'possibles' are still set using the measurements provided in the data file. The effect of this is that if only the significantly changing genes are provided in the data file, the 'observed' will equal the 'possibles'. The resulting richness calculation will not be meaningful.
If the population size is overridden using -n, the 'possibles' are still set using the measurements provided in the data file. The effect of this is that if only the significantly changing genes are provided in the data file, the 'observed' will equal the 'possibles'. The resulting richness calculation will not be meaningful.