This repository contains the data and code associated with the publication "Cryptic coral diversity is associated with symbioses, physiology, and response to thermal challenge" by Aichelman et al.
Abstract associated with the publication:
Coral persistence in the Anthropocene depends on interactions among holobiont partners (coral animals, microbial symbionts) and their environment. Cryptic coral lineages–genetically distinct yet morphologically similar groups–are critically important as they often exhibit functional diversity relevant to thermal tolerance. Additionally, environmental parameters such as thermal variability may promote tolerance, but how variability interacts with holobiont partners to shape responses to thermal challenge remains unclear. Here, we identified three cryptic lineages of Siderastrea siderea in Bocas del Toro, Panamá that differ in distributions across inshore and offshore reefs, microbial associations, phenotypic traits of holobiont partners (i.e., phenomes), and skeleton morphologies. A thermal variability experiment failed to increase thermal tolerance, but subsequent thermal challenge and recovery revealed one lineage maintained elevated energetic reserves, photochemical efficiency, and growth. Lastly, coral cores highlighted that this lineage also exhibited greater growth historically. Functional variation among cryptic lineages highlights their importance in predicting coral reef responses to climate change.
This repository is organized such that each folder contains the data (or details on where to access the data) and code needed to recreate all analyses presented in the manuscript, including the the 2bRADseq population genetic analyses, ITS2 and 16S metabarcoding analyses, and coral host and symbiont phenome analyses.
All raw sequencing files have been uploaded to NCBI's SRA (2bRAD BioProject ID=PRJNA973074, 16S/ITS2 BioProject ID= PRJNA973086).
For any questions, please contact Hannah Aichelman (hannahaichelman @ gmail.com) or Sarah Davies (daviessw @ gmail.com).
