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README

This repository contains code and information related to the paper "Scalable electron tomography for connectomics" by Kuan, Phan, et al. Questions about the code and data should be address to the corresponding authors: aaron.kuan@yale.edu, mellisman@ucsd.edu, wei-chung_lee@hms.harvard.edu

Electron Tomography Data

15 Sequential Serial Sections

Dataset shown in Fig. 2 and Extended Data Fig. 5 & 7.

Full Sampling

Limited Tilt

Restored Limited Tilt

5x5 Serial Montage

Dataset shown in Extended Data Fig. 6.

Serial Montage

Analysis Code

Tomographic Reconstruction

Tomograms were reconstructed using the TXBR package. See also Phan et al. 2012.

Fourier Shell Correlation

FSC figures were generated from the jupyter notebook "250614_plot_FSC_figs.ipynb". The FSC calculations were performed using the python script "250613_ab_calc_tomo_FSCs_2d_incr.py", which requires sub-sampled tomograms (e.g. from even-only or odd-only projections) to already be available.

Fiducial-Based Resolution Measurements

Figures relating to fiducial (gold bead) measurements were generated from the jupyter notebook "bead_resolution/250826_bead_res.ipynb". Prerequisite calculations were made using the python script "bead_resolution/250826_download_bead_data.py".

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Code for processing and evaluating TEM tomography data

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